A peptide certificate of analysis shows what an independent laboratory found in one specific batch: identity confirmed by mass spectrometry, purity measured by HPLC, and the measured quantity against the labelled amount. Read it by checking the batch reference against your vial first, then the identity result, then the purity figure and the test date.
For research purposes only. Not for human consumption. This article is educational and references published laboratory research; it is not medical advice and does not describe an approved use of any compound.
An analysis report is only useful if you can read it, and most buyers never look past the headline number. Here is what each section actually tells you, and which parts matter when you are comparing suppliers. Vitality Peps publishes a batch report for every compound it ships.
The batch reference is the whole point
A report is tied to a specific production batch. If the batch reference on the report does not match the batch on your vial, the report describes a different material. This is the first thing to check and the most commonly skipped.
The Vitality Peps lab results page lists records by batch so a vial can be matched to its report directly.
HPLC: what the chromatogram shows
High performance liquid chromatography separates the contents of a sample over time. Each component appears as a peak. A dominant single peak with minimal additional peaks indicates a sample that is largely one compound.
The reported figure is derived from the area under the main peak relative to total peak area. Read the chromatogram rather than the number alone: a report with a headline figure and no visible trace is not verifiable.
Mass spectrometry: is it the right molecule
HPLC answers how much of one thing is present. Mass spectrometry answers whether that thing is the molecule it is supposed to be, by matching the observed mass against the expected molecular weight for the sequence.
Both matter. A sample can be a very clean preparation of the wrong compound.
Test date and testing party
- Test date should be reasonably close to the production batch, not years old.
- The testing party should be named. An unattributed report is not an independent one.
- The compound name and stated vial mass on the report should match the product listing.
Questions worth asking a supplier
- Can you send the report for the specific batch I will receive?
- Does the report include the chromatogram, not just a summary figure?
- Who performed the analysis?
- How recent is the test relative to the batch?
Frequently asked questions
What purity should a research peptide have?
Reports on well-made research peptides commonly show HPLC purity above 98 percent. The number alone is not enough: the identity check by mass spectrometry confirms it is the right molecule, and the measured mass confirms the vial contains what the label states.
Is HPLC purity the same as content?
No. HPLC purity is the share of the peptide-related material that is the target compound. It says nothing about how much material is in the vial. The measured mass or net peptide content answers that, which is why both figures appear on a full report.
How do I verify a certificate is real?
Use the verification link or QR code that leads to the laboratory's own website and compare the batch reference, date and figures with the copy you were shown. Vitality Peps reports are produced by Janoshik Analytical and link to Janoshik's verification page.
What does a COA not tell you?
A standard HPLC and MS report does not cover endotoxin, sterility, residual solvents or heavy metals unless those tests were ordered separately. It also only describes the batch that was tested, not a different batch under the same product name.
For research purposes only. Not for human consumption. This article is educational and references published laboratory research; it is not medical advice and does not describe an approved use of any compound.